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CAS 4197-25-5 | Solvent Black 3

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Common NameSolvent Black 3
CAS Number4197-25-5Molecular Weight456.541
Density1.3±0.1 g/cm3Boiling Point726.0±60.0 °C at 760 mmHg
Molecular FormulaC29H24N6Melting Point120-124 °C(lit.)
MSDSChineseUSAFlash Point392.9±32.9 °C

Names

NameSolvent Black 3
SynonymMore Synonyms

Solvent Black 3 BiologicalActivity

DescriptionSudan Black B, a fat-soluble diazo dye, is a histochemical stain. Sudan Black B can be used for staining of neutral triglycerides and lipids[1].
Related CatalogResearch Areas >>OthersSignaling Pathways >>Others >>Others
In VitroSudan Black B works also in frozen material, and therefore can be complementarily used with SA-β-gal when investigating senescence. Sudan Black B is a highly lipophilic agent that exhibits high affi nity to the lipid compartment of lipofuscin. The positive Sudan Black B-lipofuscin reaction reveals blue-black intracellular granules in cells and frozen tissues and brown to black granules in FFPE tissues under the light microscope.
References

[1]. Konstantinos Evangelou, et al. Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells. Methods Mol Biol. 2017;1534:111-119.

Chemical & Physical Properties

Density1.3±0.1 g/cm3
Boiling Point726.0±60.0 °C at 760 mmHg
Melting Point120-124 °C(lit.)
Molecular FormulaC29H24N6
Molecular Weight456.541
Flash Point392.9±32.9 °C
Exact Mass456.206238
PSA73.50000
LogP7.51
Vapour Pressure0.0±2.4 mmHg at 25°C
Index of Refraction1.702
InChIKeyYCUVUDODLRLVIC-UHFFFAOYSA-N
SMILESCC1(C)Nc2cccc3c(N=Nc4ccc(N=Nc5ccccc5)c5ccccc45)ccc(c23)N1
Storage conditionStore at RT.

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :
SD4431500
CHEMICAL NAME :
1H-Perimidine, 2,3-dihydro-2,2-dimethyl-6-((4-(phenylazo)-1-naphthyl )azo)-
CAS REGISTRY NUMBER :
4197-25-5
LAST UPDATED :
199712
DATA ITEMS CITED :
4
MOLECULAR FORMULA :
C29-H24-N6

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
63 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CSLNX* U.S. Army Armament Research & Development Command, Chemical Systems Laboratory, NIOSH Exchange Chemicals. (Aberdeen Proving Ground, MD 21010) Volume(issue)/page/year: NX#04918 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M2167 No. of Facilities: 601 (estimated) No. of Industries: 10 No. of Occupations: 10 No. of Employees: 4471 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M2167 No. of Facilities: 1141 (estimated) No. of Industries: 15 No. of Occupations: 24 No. of Employees: 19262 (estimated) No. of Female Employees: 13466 (estimated)

Safety Information

Personal Protective EquipmentEyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard CodesF: Flammable;Xi: Irritant;
Risk Phrases11-36/37/38
Safety PhrasesS24/25-S16
RIDADRNONH for all modes of transport
WGK Germany3
RTECSSD4431500
HS Code32041900

Customs

HS Code32041900

Articles29

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Synonyms

(2,2-dimethyl-1,3-dihydroperimidin-6-yl)-(4-phenyldiazenylnaphthalen-1-yl)diazene
MFCD00006919
2,2-dimethyl-6-[(E)-{4-[(E)-phenyldiazenyl]naphthalen-1-yl}diazenyl]-2,3-dihydro-1H-perimidine
Sudan Black B,high purity biological stain
Fat Black HB
Sudan Black B,certified
2,2-Dimethyl-6-[(E)-{4-[(E)-phenyldiazenyl]-1-naphthyl}diazenyl]-2,3-dihydro-1H-perimidine
SOLVENT BLACK 3
1H-Perimidine, 2,3-dihydro-2,2-dimethyl-6-[(E)-2-[4-[(E)-2-phenyldiazenyl]-1-naphthalenyl]diazenyl]-
2,3-dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthyl]azo]perimidine
Ceres Black BN
EINECS 224-087-1
Sudan Black B

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