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CAS 6104-58-1 | Brilliant blue G-250

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Common NameBrilliant blue G-250
CAS Number6104-58-1Molecular Weight854.020
Density>1.0 g/cm3 (20ºC)Boiling Point/
Molecular FormulaC47H48N3NaO7S2Melting Point100 °C
MSDSUSAFlash Point11 °C
Symbol
GHS02, GHS06, GHS08
Signal WordDanger

Names

NameAcid Blue 90
SynonymMore Synonyms

Brilliant blue G-250 BiologicalActivity

DescriptionBrilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome[1][2][3].
Related CatalogResearch Areas >>OthersSignaling Pathways >>Others >>Others
References

[1]. Dyballa N, Metzger S. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels. J Vis Exp. 2009;(30):1431. Published 2009 Aug 3.

[2]. Han XX, et al. Highly sensitive protein concentration assay over a wide range via surface-enhanced Raman scattering of Coomassie brilliant blue. Anal Chem. 2010;82(11):4325-4328.

[3]. Zohny MH, et al. Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need [published online ahead of print, 2022 Feb 21]. Biomed Pharmacother. 2022;148:112723.

Chemical & Physical Properties

Density>1.0 g/cm3 (20ºC)
Melting Point100 °C
Molecular FormulaC47H48N3NaO7S2
Molecular Weight854.020
Flash Point11 °C
Exact Mass853.283142
PSA158.67000
LogP11.19820
InChIKeyRWVGQQGBQSJDQV-UHFFFAOYSA-M
SMILESCCOc1ccc(Nc2ccc(C(=C3C=CC(=[N+](CC)Cc4cccc(S(=O)(=O)[O-])c4)C=C3C)c3ccc(N(CC)Cc4cccc(S(=O)(=O)[O-])c4)cc3C)cc2)cc1.[Na+]
Storage conditionStore at RT.

Safety Information

Symbol
GHS02, GHS06, GHS08
Signal WordDanger
Hazard StatementsH225-H301-H311-H315-H319-H331-H370
Precautionary StatementsP210-P260-P280-P301 + P310-P305 + P351 + P338-P311
Personal Protective EquipmentEyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard CodesF,T,Xn
Risk PhrasesR36/37/38
Safety PhrasesS22-S24/25-S36-S26-S45-S36/37/39-S16-S9-S7
RIDADRUN 2924 3/PG 2
WGK Germany3
HS Code32041200

Customs

HS Code32041200

Articles51

More Articles
Contrast recognizability during brilliant blue G - and heavier-than-water brilliant blue G-assisted chromovitrectomy: a quantitative analysis.

Acta Ophthalmol. 91(2) , e120-4, (2013)

To evaluate the potential of heavier-than-water brilliant blue G (BBG-D(2) 0) to stain the internal limiting membrane (ILM) during chromovitrectomy.In a nonrandomized, prospective, clinical multicentr...

Reconfiguring the quorum-sensing regulator SdiA of Escherichia coli to control biofilm formation via indole and N-acylhomoserine lactones.

Appl. Environ. Microbiol. 75 , 1703-16, (2009)

SdiA is a homolog of quorum-sensing regulators that detects N-acylhomoserine lactone (AHL) signals from other bacteria. Escherichia coli uses SdiA to reduce its biofilm formation in the presence of bo...

Lipopolysaccharide inhibits the channel activity of the P2X7 receptor.

Mediators Inflamm. 2011 , 152625, (2011)

The purinergic P2X7 receptor (P2X7R) plays an important role during the immune response, participating in several events such as cytokine release, apoptosis, and necrosis. The bacterial endotoxin lipo...

Synonyms

CBB G-250
benzenemethanaminium, N-[(1Z,4E)-4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)
Coomassie Brilliant Blue G250
Benzenemethanaminium, N-[(1E,4E)-4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)
EINECS 228-058-4
Cyanine G
benzenemethanaminium, N-[(1E,4Z)-4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)
PAGE BLUE G90
coomassie briliant blue G-250
coomassie blue G-250
Sodium 3-({[(1E,4Z)-4-({4-[(4-ethoxyphenyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)amino]-2-methylphenyl}methylene)-3-methyl-2,5-cyclohexadien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate
Coomassie Brilliant blue
Polar Blue G
Blue G 250
BRILLIANT BLUE
Sodium 3-({[(1Z,4E)-4-({4-[(4-ethoxyphenyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)amino]-2-methylphenyl}methylene)-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate
coomassie brillant blue G
SERVA BLUE G
MFCD00078482
Sodium 3-({[(1E,4E)-4-({4-[(4-ethoxyphenyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)amino]-2-methylphenyl}methylene)-3-methyl-2,5-cyclohexadien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate

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