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CAS 10127-02-3 | Basic Orange 14

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Common NameBasic Orange 14
CAS Number10127-02-3Molecular Weight739.943
Density/Boiling Point468.6ºC at 760 mmHg
Molecular FormulaC34H40Cl4N6ZnMelting Point165 °C
MSDSChineseUSAFlash Point237.2ºC
Symbol
GHS08
Signal WordWarning

Names

NameBasic Orange 14
SynonymMore Synonyms

Basic Orange 14 BiologicalActivity

DescriptionAcridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3].
Related CatalogResearch Areas >>Others
In VitroGuidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[4]. 1. 用 Acridine Orange zinc chloride salt 染色细胞 (1 μM; 20 分钟; 37 ℃)。 2. 用 PBS 洗涤细胞。 3. 通过共聚焦激光扫描显微镜 (FV3000,Olympus) 观察细胞。
In VivoAcridine Orange (0.1 mg/kg,静脉注射,狗) zinc chloride salt 在 30 天内没有表现出临床毒性迹象,血液中也没有发现异常[1]。
References

[1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8.  

[2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56.  

[3]. Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925.  

[4]. Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55(1):e13172.  

Chemical & Physical Properties

Boiling Point468.6ºC at 760 mmHg
Melting Point165 °C
Molecular FormulaC34H40Cl4N6Zn
Molecular Weight739.943
Flash Point237.2ºC
Exact Mass736.135986
PSA38.74000
LogP10.02050
InChIKeyVADJQOXWNSPOQA-UHFFFAOYSA-L
SMILESCN(C)c1ccc2cc3ccc(N(C)C)cc3nc2c1.Cl.Cl[Zn]Cl
Storage conditionStore at RT.
Water Solubilitysoluble

Safety Information

Symbol
GHS08
Signal WordWarning
Hazard StatementsH341
Precautionary StatementsP281
Personal Protective EquipmentEyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard CodesXn:Harmful;
Risk PhrasesR20/21/22;R68
Safety PhrasesS45-S36/37
RIDADRNONH for all modes of transport
WGK Germany3
RTECSAR7600000
Packaging GroupI; II; III
Hazard Class6.1
HS Code32041300

Customs

HS Code32041300

Articles17

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Synonyms

N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc (2:1) dihydrochloride
Acridine Orange hemi(zinc chloride) salt
MFCD00081043
zinc,3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine,dichloride,hydrochloride
Acridine Orange
N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc hydrochloride (2:1:2)
Euchrysin GNY
EINECS 200-614-0

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