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| OEM | OEM Services Provided |
| Feature | Good stability,Easy to Use |
| Classification | Dyes and Pigments |
| Advantage | Professional, Fast Delivery, Customizable | Samples | Apply for free，Contact us now. |
| Product Name: | Toluidine Blue O |
| Synonyms: | DIMETHYLTOLUTHIONINE CHLORIDE;CI NO 52040;CI BASIC BLUE 17;CI 52040;fklot;gabilin;klot;menodin |
| CAS: | 92-31-9 |
| MF: | C15H16ClN3S |
| MW: | 305.82 |
| EINECS: | 202-146-2 |
| Product Categories: | Dyes and Pigments;Phenothiazine;TOLAZUL;pharmacetical |
| Mol File: | 92-31-9.mol |
| Melting point | >200℃ |
| bulk density | 410kg/m3 |
| storage temp. | Inert atmosphere,Room Temperature |
| solubility | H2O: soluble1mg/mL |
| form | Solid |
| pka | 2.4, 11.6(at 25℃) |
| Colour Index | 52040 |
| color | Dark green to black |
| PH | 2.8 (H2O, 25℃) |
| Water Solubility | 3.82 g/100 mL |
| λmax | 626 nm, 630 nm |
| ε(extinction coefficient) | ≥13000 at 241-247nm in H2O ≥28000 at 287-293nm in H2O ≥40000 at 625-635nm in H2O |
| Merck | 14,9520 |
| Stability: | Stable. Incompatible with strong oxidizing agents. |
| Biological Applications | Bound hyaluronan assay; DNA assay; bioelectronic applications; biofuel cells; microbial fuel cells; detecting bacteria,body fluid,nucleic acids,45,proteins,viruses; identifying Gram-negative bacteria,b-amyloid reducing agents |
| EPA Substance Registry System | Tolonium chloride (92-31-9) |
| Risk Statements | 36/37/38 |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
| RTECS | SP5670000 |
| TSCA | Yes |
| HS Code | 32041300 |
| Toxicity | LD50 in mice, rats, rabbits (mg/kg): 27.56, 28.93, 13.44 i.v. (Haley, Storlarsky) |
| Provider | Language |
|---|---|
| 3-Amino-7-dimethylamino-2-methylphenazathionium chloride | English |
| SigmaAldrich | English |
| ACROS | English |
| Chemical Properties | Toluidine Blue O is a dark green or black-gray powder with bronze luster. It is soluble in water (3.82g/100ml), producing a blue-violet solution, slightly soluble in ethanol (0.57g/100ml) to give a blue solution, very slightly soluble in chloroform, and almost insoluble in ether. | |||||||||||||||||||||
| Blutene,Abbott,US,1953 | ||||||||||||||||||||||
| Uses | Toluidine Blue O is a metachromatic dye suitable for a wide variety of histological staining procedures. It is used for direct dyeing and printing of wool and silk, as well as a biological stain and hemostatic agent. | |||||||||||||||||||||
| ChEBI: Toluidine Blue O is an organic chloride salt having 3-amino-7-(dimethylamino)-2-methylphenothiazin-5-ium (tolonium) as the counterion. It is a blue nuclear counterstain that can be used to demonstrate Nissl substance and is also useful for staining mast cell granules, both in metachromatic and orthochromatic techniques. It has a role as a fluorochrome, a histological dye and a diagnostic agent. It contains a tolonium. | ||||||||||||||||||||||
| Preparation | by heavy chromate salt in the presence of sodium content and deal with N1,N1-dimethylbenzene-1,4-diamine, transforming it into the content and sulfonic acid, this product then through heavy chromate salt and O-Methylaniline processing, into a unique of sulfur acid amine generation, then use heavy chromate salt and copper sulfate closed loop and oxidation, Finally,?the?zinc chloride?salt?precipitation | |||||||||||||||||||||
| As taken from US Patent 416,055 (probably the oldest patent on themanufacture of a currently-used drug): In carrying out this process about 6pbw of dimethyl-p-phenylenediamine was dissolved in about 18 pbw ofhydrochloric acid of about 1.16 specific gravity and then a solution of about3.8 pbw of nitrite of soda in about 6 pbw of water was gradually added. Thehydrochlorate of nitroso-dimethylaniline thus produced in the well-knownmanner is then submitted to the reducing action of zinc-dust by adding, firstabout 30 pbw of hydrochloric acid of about 1.16 specific gravity and then (insmall portions at a time) about 10 pbw of zinc-dust as is well understood bychemists. The solution of hydrochlorate of paramido-dimethylaniline thusobtained is afterwards diluted with about 250 pbw of water and then theuncombined hydrochloric acid contained in the solution is, if any, neutralizedby the addition of an alkali. There are then added about 16 pbw of sulfate ofalumina and about 13 pbw of thiosulfate of sodium, (hyposulfite of soda) andimmediately afterwards a solution of about 5 pbw of bichromate of potash inabout 60 pbw of water is quickly run in. In this stage of the process the formation of an acid sulfureted compound ofparamidodimethylaniline takes place, possessing the formulaC8H11N2SSO3H(paramido-dimethylaniline-thiosulfonic acid). Without previousseparation of this intermediate compound a solution of about 5.3 pbw oforthotoluidine, in the requisite amount of dilute hydrochloric acid (about 6pbw of hydrochloric acid, SG about 1.16, diluted with about 6 pbw water) andshortly afterwards a solution of about 14 pbw of bichromate of potash inabout 160 parts by weight of water is then added under constant agitation,when a precipitate will be formed chiefly consisting of a green indaminepossessing in its dry condition the formula C15H17N3S2O3. In order totransform the same into toluidine-blue, about 50 pbw of a solution of chlorideof zinc of about 1.5 specific gravity are added and the mixture thus obtainedis boiled during about half an hour, when, after cooling, the toluidine-blue thusformed will separate out and may then be filtered and purified, if necessary,by repeated solution in water and precipitation by means of chloride of sodiumand chloride of zinc. In the above described process the sulfate of alumina may be dispensed withand replaced by as much hydrochloric, sulfuric, or acetic acid as will berequired to liberate the thiosulfuric acid from the thiosulfate of sodiumemployed. Toluidine-blue prepared as above described presents the followingcharacteristic properties: It consists principally of the hydrochlorate of dimethyltoluthionine, the composition of which corresponds to the formulaC15H15N3SHCl. | ||||||||||||||||||||||
| Therapeutic Function | Coagulant | |||||||||||||||||||||
| Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin. | ||||||||||||||||||||||
| Staining Procedures | The staining solution is prepared by dissolving 0.05% toluidine blue O in buffer, pH 4.4-6.8. Sections or whole tissues are stained for 5-10 min followed by differentiation using the same buffer until excess stain is removed. Cellulose and other glucans become red-violet depending on pH, lignified elements and other polyphenols become bluish-green. Fungal hyphae are often distinguished from the surrounding host tissue because of the difference in cytoplasmic pH. | |||||||||||||||||||||
Soluble in water for blue purple, soluble in ethanol to blue. The strong sulfuric acid for yellow green, diluted to blue. The dye solution to join sodium hydroxide have dark purple precipitation.
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| Raw materials | N,N-Dimethyl-1,4-phenylenediamine-->o-Toluidine-->Sodium nitrite-->Zinc chloride-->Sodium thiosulfate-->o-Toluidine-->N,N-Dimethyl-1,4-phenylenediamine |
Declaration: The products displayed on this website are intended exclusively for industrial applications or scientific research. They are not intended for medical, pharmaceutical, or food use. In accordance with applicable laws and regulations, purchasing organizations must hold valid qualifications and approvals.
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