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Acid Blue 83 | CAS 6104-59-2

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Acid Blue 83 Basic information

Product Name:Acid Blue 83
Synonyms:SERVA BLUE R;SERVA BLUE R STAINING SOLUTION;SOLAR CYANINE 6B;R STANDS FOR ''REDDISH'';PAGE BLUE 83;Coomassie brilliant R250;Benzenemethanaminium, N-[4-[[4-[(4-ethoxyphenyl) amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino ]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]- N-ethyl-3-sulfo-, hydroxide, inner salt, monosodium salt;Brilliant Blue 6B
CAS:6104-59-2
MF:C45H46N3NaO7S2
MW:827.99
EINECS:228-060-5
Product Categories:Dyes and Pigments;Organics;Biochemistry;Reagents for Electrophoresis
Mol File:6104-59-2.mol

Acid Blue 83 Chemical Properties

Melting point 174-180 °C
bulk density250kg/m3
vapor density 28.5 (vs air)
Fp 14 °C
storage temp. room temp
solubility H2O: soluble10mg/mL
form Powder/Solid
Colour Index 42660
color Red Brown
PH6.2 (25°C, 10g/L in H2O)
Water Solubility Soluble in water.
λmax589.2 nm in ethanol
BRN 5718025
Stability:Light Sensitive
InChIKeyNKLPQNGYXWVELD-UHFFFAOYSA-M
CAS DataBase Reference6104-59-2(CAS DataBase Reference)
EPA Substance Registry SystemC.I. Acid Blue 83 (6104-59-2)

Safety Information

Hazard Codes 
Risk Statements 
Safety Statements 
WGK Germany 3
TSCA Yes
HS Code 32041200

MSDS Information

ProviderLanguage
Acid Blue 83 English
ACROS English
SigmaAldrich English

Acid Blue 83 Usage And Synthesis

UsesPreparationstorage
Chemical Propertiesdark bordeaux to dark purplish-brown cryst. powder
Acid Blue 83 is a dye commonly used in laboratories or quantify proteins.
UsesBrilliant Blue R is used for detection of protein bands following electrophoresis. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100 ng/band. It may be combined with other stains, such as silver stain, to distinguish different types of proteins. It displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1.
p-Chlorobenzaldehyde?(1 Moore) and 3-(Ethyl(phenyl)amino)methyl)benzenesulfonic acid (2 Moore) condensation, and heavy chromate salt-oxalic acid oxidation its product, again with 4-Ethoxybenzenamine condensation.
Biochem/physiol ActionsBrilliant Blue R is a methanol based stain. It can also stain agarose gels. It consists of methanol and acetic acid.
Room temperature
Properties and Applicationsblue. Dark blue powder. Not soluble in cold water, slightly soluble in hot water and ethanol for light blue. The strong sulfuric acid for orange red, diluted into blue. In water solution to join sodium hydroxide purple precipitation. Used for wool, silk, polyamide fiber fabric dyeing and printing directly, can also be used for leather and feathers dyeing.
Standard Light Fastness Soaping Persperation Fastness Oxygen bleaching Fastness to seawater
Fading Stain Fading Stain Fading Stain
ISO 3 4 4 4 1 1-2 4 4-5
AATCC 2-3 3-4 3-4 4-5 1 1-2 4-5 4-5

Acid Blue 83 Preparation Products And Raw materials

Raw materials4-Chlorobenzaldehyde-->Potassium dichromate-->Benzenesulfonic acid-->Phenetidine-->4-((Ethylanilino)methyl)benzenesulphonic acid-->Phenetidine-->4-Chlorobenzaldehyde-->N-Ethyl-N-benzylaniline-3'-sulfonic acid

Declaration: The products displayed on this website are intended exclusively for industrial applications or scientific research. They are not intended for medical, pharmaceutical, or food use. In accordance with applicable laws and regulations, purchasing organizations must hold valid qualifications and approvals.

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